Department of Physiology, Tufts University School of Medicine
136 Harrison Ave., Boston, Massachusetts, 02111, USA
Lab and Office: Jaharis Building, Room 708
Professor of Physiology
PHONE: (617) 636-0442 (office), (617) 636-6744(lab)
FAX: (617) 636-6745
Former lab members
Jay Lee, MD/PhD Student (grad 2002)
Dae-Won Kim, Graduate Student (grad 2000)
Deepa Bhavsar, Postdoctoral Fellow
Joan Abrams Graduate Student(grad 2000)
My lab is broadly interested in how growth factors such as PDGF regulate cell growth. For several years we have been working on how the c-fos promoter is regulated. We discovered a growth factor inducible called SIF which turns out to be composed of STAT family transcription factors. We are currently interested in the role of STAT3 in cancer and in cancer stem cells in particular. We have been studying this primarily in the context of the glioblastoma brain cancer. The graphic at the top of the page shows cancer stem cells from a human glioblastoma growing as neurospheres on the left and stained with stem cell and neural markers. Another interest of the lab is the development of an annotated database of genes called MedStract in collaboration with a computational linguistics group at Brandeis. We have also pioneered the use of a human near haploid cell line for somatic cell genetics.
Click for a list of projects ongoing.
Role of STAT3 in gioblastoma stem cells
Mechanism of activation of Stats by PDGF
Regulation of c-fos by the Jak/Stat pathway
Cell cycle regulation of growth factor signalling
Activation of STATs by oxidative stress
Development of human haploid cells as a genetic system.
Role of Cdc37 in signal transduction and the cell cycle
Role of TFII-I in c-fos regulation and signal transduction
Development of the annotated gene database called MedStract
Carette JE, Guimaraes CP, Varadarajan M, Park AS, Wuethrich I, Godarova I, Kotecki M, Cochran BH, Spooner E, . Ploegh HL, and . Brummelkamp TR. 2009. Haploid Genetic Screens in Human Cells Identify Host Factors Used by Pathogens. Science 326:1231-5. Abstract , pdf
Sherry MM, Reeves A, Wu JK, Cochran BH. 2009. STAT3 is required for proliferation and maintenance of multipotency in glioblastoma stem cells. Stem Cells, 27:2383 - 2392. abstract, pdf
Konnikova L, Simeone MC, Kruger MM, Kotecki M, Cochran BH. 2005. Signal transducer and activator of transcription 3 (STAT3) regulates human telomerase reverse transcriptase (hTERT) expression in human cancer and primary cells. Cancer Res. 65(15):6516-20. Abstract
Konnikova L, Kotecki M, Kruger MM, Cochran BH. 2003. Knockdown of STAT3 expression by RNAi induces apoptosis in astrocytoma cells. BMC Cancer 2:23. free full text, pdf
Simon AR, Takahashi S, Severgnini M, Fanburg BL, Cochran BH. 2002. Role of the JAK-STAT pathway in PDGF-stimulated proliferation of human airway smooth muscle cells. Am J Physiol Lung Cell Mol Physiol Jun;282(6):L1296-304. abstract, full text
Kim, D.-W. and B.H. Cochran. 2001. Jak2 activates tfii-i and regulates its interaction with extracellular signal-regulated kinase. Mol Cell Biol. 2001 May 15;21(10):3387-97. abstract, pdf
Simon AR, Vikis HG, Stewart S, Fanburg BL, Cochran BH, Guan KL. 2000. Regulation of STAT3 by direct binding to the rac1 GTPase. Science 2000 Oct 6;290(5489):144-7. Abstract, pdf
Kim, D.-W. and B.H. Cochran. 2000. ERK binds to TFII-I and regulates its activation of the c-fos promoter. Mol. Cell Biol. 20(4):1140-8. abstract, pdf
Kotecki, M., P. S. Reddy, and B. H. Cochran. 1999. Isolation and characterization of a near-haploid human cell line. Exp Cell Res, Nov 1;252(2):273-80. abstract, pdf
Grammatikakis, N., Lin, J.-H., Grammatikakis, A., Tsichlis, P. N. and Cochran, B. H. (1999). p50cdc37 acting in concert with Hsp90 is required for Raf-1 function. Mol. Cell Biol., 19, 1661-72. abstract, pdf
Kim, D.-W., Cheriyath, V., Roy, A. L. and Cochran, B. H. (1998). TFII-I enhances activation of the c-fos promoter through interactions with upstream elements. Mol. Cell. Biol. 18, 3310-3320.abstract, pdf
Simon, A, Rai, U., Fanburg, B. Cochran BH. 1998. Activation of the JAK/STAT pathway by reactive oxygen species. Am. J. of Physiology,44, C1640-C1652. abstract, (Download full text PDF file)
Hayes TE, Kitchen AM, Cochran BH. 1987. Inducible binding of a factor to the c-fos regulatory region. Proc. Natl. Acad. Sci. (USA) 84: 1272-1276.
Sengupta P, Cochran BH. 1991. MATalpha1 can mediate gene activation by a-mating factor. Genes and Dev. 5: 1924-1934.
Meyer, D. J., Campbell, G., Cochran, B. H., Argetsinger, L., Larner, A. C., Finbloom, D. S., Carter-Su, C. and Schwartz, J. 1993. Growth hormone induces a DNA-binding factor related to the interferon-stimulated 91-kDa transcription factor. J. Biol. Chem. 269, 4701-4704.
Physiology Dept.**** Health Science Campus
visits since August 7, 2005